ES Cell-derived Neuroepithelial Cell Cultures
نویسندگان
چکیده
منابع مشابه
Specific microRNAs modulate ES cell-derived neurogenesis
MicroRNAs (miRNAs) are recently discovered small non-coding transcripts with a broad spectrum of functions described mostly in invertebrates. As post-transcriptional regulators of gene expression, miRNAs trigger target mRNA degradation or translational repression. While hundreds of miRNAs have been cloned from a variety of mammalian tissues and cells, and multiple mRNA targets have been predict...
متن کاملCancer prevention with semi-allogeneic ES cell-derived dendritic cells.
Dendritic cells (DC) genetically modified to present tumor-associated antigen are a promising means for anti-cancer immunotherapy. By introducing expression vectors into ES cells and subsequently inducing differentiation to DC (ES-DC), we can generate transfectant DC expressing the transgenes. In the future clinical application of this technology, the unavailability of human ES cells geneticall...
متن کاملES-Cell Derived Hematopoietic Cells Induce Transplantation Tolerance
BACKGROUND Bone marrow cells induce stable mixed chimerism under appropriate conditioning of the host, mediating the induction of transplantation tolerance. However, their strong immunogenicity precludes routine use in clinical transplantation due to the need for harsh preconditioning and the requirement for toxic immunosuppression to prevent rejection and graft-versus-host disease. Alternative...
متن کاملAn ES cell caution
ny cell therapy risks introducing mutated and possibly tumorigenic cells. Now results from Peter Stambrook (University of Cincinnati, Cincinnati, OH) and colleagues underline this concern for embryonic stem (ES) cells, which are one possible source for therapeutic cells. Stambrook constructed a cell line in which he could detect loss or mutation of a heterozygous marker gene. Loss (rather than ...
متن کاملEnhanced Proliferation of Monolayer Cultures of Embryonic Stem (ES) Cell-Derived Cardiomyocytes Following Acute Loss of Retinoblastoma
BACKGROUND Cardiomyocyte (CM) cell cycle analysis has been impeded because of a reliance on primary neonatal cultures of poorly proliferating cells or chronic transgenic animal models with innate compensatory mechanisms. METHODOLOGY/PRINCIPAL FINDINGS We describe an in vitro model consisting of monolayer cultures of highly proliferative embryonic stem (ES) cell-derived CM. Following induction...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2006
ISSN: 1940-087X
DOI: 10.3791/118